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The small man with carefully brushed long hair and tinted glasses sat quietly as the band played a competent version of "Little Red Rooster".
Jacobson and Glazer aren't the first comedians to impersonate some younger, poorer, less competent version of themselves (see Lucy, Liz Lemon, Louie), but sometimes it can be hard for viewers, to say nothing of the comedians themselves, to differentiate.
In the case of embryotoxicity, one could imagine complementing the envisioned metabolically competent version of the EST by testing the identified metabolites on zebrafish or hen eggs.
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In the grand TV-sitcom tradition, Jacobson and Glazer play less driven, less competent versions of their younger selves.
"The next day, we ended up throwing together some, I'm sure, sub-competent version of '(You Caught Me) Smilin', ' and did a whole sound-collage thing".
A replication-competent version of Ad26 (rcAd26) was constructed by adding the Ad26 E1 region 5′ of the transgene cassette in the replication-incompetent version of our Ad26 vector (16).
Interestingly, although the induction of Noxa in the p65-reconstituted cells was completely ablated by DN-p73β, the ectopic expression of a transactivation-competent version of p73β failed to activate expression of Noxa in the p65-null cells.
The N-myristoylation-competent version scFvG2/p17 carried a sequence which started with the N-terminal dipeptide Met-Gly, while the N-myristoylation-incompetent version scFvE2/p17 started with Met-Glu.
We have previously shown that replication-competent versions of a deleted version of CPMV RNA-2 (delRNA-2)[24] can be used to produce assembled IgG molecules in plants [25].
Leaky expression from adenoviral promoters is an issue in the application of adenoviral vectors as gene delivery vehicles, and recombination with wt adenovirus may transform replication-deficient, E1- and E3-deleted adenoviral vectors into replication-competent versions.
The HSV-TK expression unit of the cassette, in conjunction with the amiRNA expression unit, may also help to bring any unforeseen high-level replication of adenoviral vectors under control, should they turn into replication-competent versions (i.e., upon unintended recombination with a wt virus).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com