Sentence examples for competent to transform from inspiring English sources

For example, an inflammatory response is required to induce cancers in chickens infected with the potent oncogenic Rous Sarcoma virus despite it carrying the v- src oncogene that alone is competent to transform fibroblasts in culture (Sieweke et al, 1989).

Taking all data together from this work and others, LARs from different plants have displayed competent biochemical activity to transform leucoanthocyanidins to trans-flavan 3-ols in vitro; however, the lack of accumulation of trans-flavan 3-ols and their derivatives accompanying expression of LAR in heterologous LAR-free plants still raises questions.

The only modification of the protocol is that BL21 competent cell was used to transform the recombination PCR product and all transformation products were cultured on Amp-LB plate containing 2 mM IPTG.

A total of 500 ng of BglII-digested pLAG6 or pLAG10 was used to transform competent yeast cells, selecting for Ura+ transformants.

A total of 500 ng of linearized CFVs pLS192 and pLAG8, digested with SnaBI and BglII, respectively, was used to transform competent yeast cells, selecting for Ura+ transformants.

The TOPO TA cloning kit (Invitrogen) was employed to clone the cDNA fragment in a suitable vector (PCR II-TOPO plasmid), which was then used to transform competent Escherichia coli cells (One Shot Top 10 Chemically Competent E. coli).

ECOS X Competent E. coli DH5α (Nippon gene, Tokyo, Japan) chemically competent cells were used to transform the recombinant DNA generated using the SLiCE method, in order to obtain the constant transformation efficiency.

The vectors were used to transform competent DH10Bac E. coli cells.

Regardless the nature of this plasmid mixture, it was totally used to transform competent E. coli JM109 DE3) cells.

The plasmid was used to transform competent E. coli DH10 Bac cells, and the extracted bacmid was used to transfect Sf9 cells using the Cellfectin II Reagent (Invitrogen).

The purified product was ligated into the pGEM®-T Easy vector (Promega) and the resulting plasmids were used to transform competent E. coli JM109 cells.