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It has been demonstrated that S. aureus invades and survives within mammalian host cells and is competent to replicate in the phagosome or escape the phagosome and persist within the host cells, which can induce anti-apoptotic programs in phagocytes [ 2].
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One main concern is the possibility that reconstruction of standard virus by recombination between two defective genomes would generate a competent virus able to replicate and cause disease.
The first incubation was done to replicate competent bacteria on a large scale.
The shuttle vector and viral packaging system were cotransfected into HEK293T cells to replicate competent lentivirus.
So pGCSIL/Id1-3 vector and viral packaging system (Gikai gene company, Shanghai, China, containing an optimized mixture of two packaging plasmids: pHelper 1.0 vector and pHelper 2.0 vector) were cotransfected into 293 cells to replicate competent lentivirus.
In addition, the differences in the quality of those processes and in performance persist over time, suggesting that competent management is not easy to replicate.
Also in the immune competent mice, the mutant failed to replicate immediately after infection.
Secondly, with the possible exception of an as-yet-undiscovered replication-competent member, the inability of all HERVs to replicate would also violate the second of Koch's postulates.
Non inactivated virus has been postulated to replicate in equids to high titers and to be infectious for the local populations of competent and capable mosquito vectors.
Try to replicate it.
"It's hard to replicate".
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