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For F3 cells to be a suitable vehicle for the generation of transgenic mice they should be competent to generate chimeric mice that can transmit alleles through the germline.
These iPS cell clones appear to be equivalent to embryonic stem cells in gene expression and are competent to generate germline chimeras.
In this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors.
On the other hand, Sacchetti et al. have shown that osteoblasts alone cannot recapitulate a hematopoietic niche upon transplant, and implicated a subpopulation of bone marrow stromal cells, the subendothelial cells within sinusoidal walls in marrows, as competent to generate osteogenic progenitors and to recapitulate a hematopoietic marrow upon transplant [43].
To assess whether the lack of an ex vivo response in HuD+/+ mice was due to ineffective immunization, splenocytes stimulated in vitro with p321 were assayed and found to be competent to generate activated HuD p321-specific CD8+ T cells (Fig 3d).
In summary, neuronal progenitors are competent to generate neurons upon exposure to Tgfβ over the entire culture period, where upon a minimal time frame of 2 3 days is necessary to detect changes in the expression of differentiation markers.
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PCR conditions were as follows: five cycles of denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 45 s; and final extension at 72°C for 10 min. The PCR products were ligated into the pMD-18T vector (TaKaRa Biotechnology Co). and then transformed into Escherichia coli DH5α competent cells to generate the microsatellite sequence enriched library.
"Twenty years is long enough for a competent investment company to generate satisfactory results, especially when the market over the same period has done well and the investment was supposed to be broad based and for growth".
Aliquots of 5 μl digested PCR products were chemically transformed into 100 μl competent E.coli DH5α to generate recombinants.
Although the glycolytic phenotype in cancer cells was proposed to be due to defective mitochondrial OXPHOS, many cancer cells exhibit competent OXPHOS activity capable to generate ATP [99].
To reconstitute the SUMOylation system in vitro, pETSAE1a2 was co-transformed with pACYCDuet-AtSCE1-AtSUMO1 or pACYCDuet-AtSUMO1 into Escherichia coli BL21 (DE3) cells to generate competent cells [ 59, 60].
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