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Huh7 cells were initially transfected with the pCH-9/3091 HBV replication competent target plasmid [ 31].
Taken together, these results indicate that PEMs are probably the competent target tissue for coffee genetic transformation in C. arabica.
Further experiments are needed to determine whether the predicted miR211 complementary viral sequence is not a competent target or if synthetic miR211 is not efficiently employed as a guide RNA by RISC.
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The initial RMCE-competent target cassette, pCMV-F3-gfp-F, is controlled by a CMV promoter upstream of the FRT3 site resulting in a promoter trap as described in Qiao et al. (2009) (Fig. 1, step 1).
FISH analysis with gtn-specific probes evidenced a single integration site of the RMCE-competent targeting cassette within the DUKX-B11 F3/F host (Online resource 2).
These indirect effects could be due to nonproductively infected cells, exposure of virus particles (both defective and replication competent) to target and non-target cells as well as cellular and viral factors released from the infected cells that could affect bystander cell population.
It is believed that after the onset of this appropriate tumor immune response, the immune system becomes competent to target onconeural antigen-expressing neurons.
Only such coordinated action, as well as competent strategies targeting prevention, will be effective in reducing the burden and improving the clinical outcome of this devastating complication of diabetes.
He looks competent and businesslike.
A variety of competent ligands and target agents conjugated onto nanoparticle surfaces, including monoclonal antibodies, transferrin, and folic acid, have been used to target cell surfaces [22, 23, 24].
After the vector is readily prepared and transformed into the competent cells, the target gene can be inserted into the Pichia genome in high efficiency via homologous recombination to generate stable cell lines, and then the colonies with multiple copies that exhibit the highest protein expression level will be screened out through zeocin-spread plates (Daly and Hearn, 2005).
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