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In addition, it can also facilitate the co-transmission of various competent resistance determinants among different pathogens.
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3) After acquiring the penicillin-resistance gene, originally competent isolates might lose genetic components important for competence during high frequency of genetic transformation, resulting in isolates that are not competent.
Mr Serdyukov was a competent administrator, able to overcome resistance to change.
This was referred to as the 'calcium chloride' (CaCl2) method [ 5] and was subsequently adopted to impart antibiotic resistance into the competent E. coli.
The findings reveal how 'management techniques are co-opted into professional work as a form of resistance, with professionals being competent in management practice, rather than being co-opted into management roles' [ 42], p. 774.
The AV reovirus S1 plasmid was transformed into Top10 Escherichia coli-competent cells and screened by ampicillin resistance.
Transformation was measured by providing competent cells with a cloned allele conferring resistance to the antibiotic novobiocin (nov).
Finally, a competent electroless Ni P SiC composite plating process producing a high wear resistance and sound corrosion resistance of the coatings is obtained.
After annealing, ligation and transformation of the resulting DNA vector into competent DH5α bacteria (Invitrogen, Cergy Pontoise, France), antibiotic resistance was used to select the positive bacterial colonies.
BAC: bacterial artificial chromosome; BIBAC: plant-transformation-competent binary BAC; QTL: quantitative trait locus; RDR: resistance to Didymella rabiei; DTF: days to first flower; SSR: simple sequence repeat; CB: consensus band; EST: expressed sequence tag.
CupA localizes to the plasma membrane via a single-pass N-terminal transmembrane (TM) helix, and a Cu(I -binding competent, membrane-localI -bindingis obligatory for competentcopper resistance.
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