The ready inhibition of PBP4 by β-lactams compared to PBP5 also appears to be related to the weaker interactions holding key residues in a catalytically competent position.
Calcium binding at Ca2 is critical for triggering the movement of the active site cysteine into a catalytically competent position.
However the higher activity of the Tyr204 mutants observed at low pH is inconsistent with the proposal that such a tyrosinate ion acts to force IPP into a catalytically competent position, but rather backs up the theory that the stabilization of the PPi leaving group facilitated by the tri-nuclear Mg cluster helps drive the reaction [12].
Together, these observations depict a three-position metal switch differentially operated by Mg2+ and Ca2+, including an AMPylation-competent position where Mg2+ binds in the presence of the ATP cofactor, a deAMPylation-competent position where Ca2+ and Mg2+ bind in the presence of an AMPylated substrate, and an AMPylation inhibitory position where Ca2+ binds in the presence of ATP.
In contrast, they superpose well to cofactors bound in a position competent for PTM transfer8,9 (Fig. 1e).
Former Housing Secretary Samuel R. Pierce Jr. has told investigators that he was forced to hire a longtime neighbor of Senator Alfonse M. D'Amato as the Reagan Administration's senior housing official in New York even though "she was not competent for the position," according to a Congressional report made public today.
Think about which skills will make you more competent in the position you're applying for.
"It's not that we don't have competent people in positions of authority," Mr. Cale, 50, said in a follow-up interview.
This is similar to the double bind that women experience when ascending to leadership positions: competent and assertive women, who fail to meet the gender role expectation of being kind and empathetic, tend to be evaluated negatively.
Obama has fielded a wide-ranging zone defence, putting trusted, competent people in positions of responsibility, giving them enough direction to keep them actively supporting each other but enough freedom for them to improvise if the ball lands in their hands.
Er, not after we throw their whole army out of work, disqualify most competent Iraqis from positions of responsibility, and wreck their infrastructure.
