Sentence examples for competent medium from inspiring English sources

To induce a second recombination event for removal of the integrated plasmid, the resultant strain was cultured in competent medium containing 1 mM IPTG.

For expression profile analyses, B. subtilis 168 was grown in 13 different time-series growth conditions: anaerobic growth; competent medium; cold-shock experiments; DSM medium; DGG medium; glucose-limited medium; heat-shock experiment; LB medium; minimum-glucose medium; sodium-shock conditions; phosphate-starvation medium; and SOS stress experiments.

At a workshop in Santa Fe, N.M., Mr. Johnson learned of three steps for becoming a successful artist, ascribed to Horton Foote: Be competent at your medium, understand the history of your medium and come from a place.

Cells were grown in the above medium and competent cells were prepared similar to E. coli MOS Blue cells with the variation that washing and suspension of the cells were done in 10% glycerol with 0.1% Tween.

Also, countries should consider rectifying shortages in higher level workforce (including specialist midwives, neonatal nurses, obstetricians, paediatricians and neonatologists) as this core group is essential for building capacity of a competent workforce for the medium and long term.

For example, bacteria have been observed to reach competence (i.e. obtain the ability to kill their non-competent siblings) in rich medium in vitro in early logarithmic growth, which is not seen in vivo, where nutrients are generally limited [ 41, 42].

The last two paintings are the most accomplished in the exhibition, and rank among the best in any medium that this competent if sadly lackluster artist ever made.

This demonstrates that hESCs in 4i medium are highly competent for the hPGCLC fate.

To perform microscopy, the B. subtilis cells were grown to the competent state in 1× MC medium, collected by centrifugation (3 min, 6000 g ), resuspended in 1× PBS and mounted between glass slide and coverslip.

Bacillus subtilis 168 is easily made competent by growing it in competence starvation medium.

Both plasmids were individually transformed into E. coli JM109 competent cells for multiplication on SOB medium with Amp (100 μg/mL) at 37 °C for 24 h.