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This difference might be due to the effect of recombination, since P. fluorescens was shown to be naturally competent for transformation [ 28], whereas natural competence is unknown in S. maltophilia.
Staphylococcus aureus, including MRSA, are not competent for transformation, but do contain a few of the known competence genes (ComC, ComGA, ComGB, ComGC) (Table 4).
H. pylori is naturally competent for transformation.
Transformation appears to be common among bacterial species, and thus far at least 60 species are known to have the natural ability to become competent for transformation.
Many bacteria are naturally competent for transformation by spontaneous uptake of DNA from their surrounding environments [1], [2].
H. pylori is naturally competent for transformation by exogenous DNA, and recombination during mixed infections of one stomach with multiple H. pylori strains generates extensive allelic diversity.
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The PCR products from both subtractions were cloned into pCR 2.1® TOPO plasmid using TOP10 One Shot ® competent cells for transformation (Invitrogen).
In brief, a mix of PCR product, In-Fusion HD Enzyme Premix, pGEMHE vector was incubated at 50°C for 15 min. One microliter of the reaction was added to Stellar™ competent cells for transformation.
It is interesting to note that the two RecAs with the highest ATPase activities are S. pneumoniae and N. gonorrhoeae, both of which are naturally competent for DNA transformation.
Almost all genes previously identified in the two models (com and pil genes) have been found in A. baumannii strain AYE whereas their counterparts are either absent or disrupted in strain SDF, suggesting that the latter strain is not competent for natural transformation (Table 2).
R. solanacearum is naturally competent for DNA transformation [ 28- 30] which allows cells to acquire foreign genetic material directly from their environment.
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