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Altogether the results indicate that neutral membranes are able to interact with Penetratin which confirms that the phosphate negatively charged groups exposed on the external leaflet of plasma membrane are competent for interaction with the basic peptide.
This suggests that the scanning state should be competent for interaction with the SRP receptor, potentially permitting targeting while the TMD is still inside the tunnel.
Thus, displacement of the Ubl domain by phosphoubiquitin binding may lead to exposure of a surface competent for interaction with ubiquitin.
However, HER2 and HER3 are not able to stimulate cell signalling as monomers as HER2 does not possess an ectodomain competent for interaction with any ligand, whereas HER3 lacks the kinase complex [ 7].
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The likely role of Thr171 phosphorylation is to orient these five residues so their side-chains are in a binding competent mode for interaction with an unidentified RNA processing factor.
Experimental data show that zwitterionic lipid headgroups take part in the interaction with Penetratin suggesting that the external leaflet lipids of cells plasma membrane are competent for peptide interaction in the absence of net negative charges.
The host chaperone complex associates with DP and HP and is required to establish and maintain the polymerase in a conformation competent for ε interaction in a dynamic process driven by ATP hydrolysis.
We speculate that only oligomeric forms of PAPC transmembrane domains are competent for its interactions with FLRT3 transmembrane domain.
Our present work on the free Pfu-SRP19 supports the idea that this subunit acts as a molecular scaffold and a chaperone, assisting the SRP RNA in adopting the conformation required for its optimal interaction with the essential subunit SRP54 and thus ensuring the proper maturation and assembly of a functional SRP competent for protein-targeting through the interaction with its cognate receptor.
Early experiments with CTX inhibition of the BK channel revealed that CTX binds to the extracellular surface of the channel with a 1 1 channel:toxin stoichiometry, that both the open and closed states of the channel are competent for toxin binding, and that electrostatic interactions play an important role in enhancing the toxin's affinity (Anderson et al., 1988).
We conclude that despite asymmetry in the ligand-receptor interaction, both sides are competent for signaling, and appear to signal equally.
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