This PagP construct, initially reported by Burgess et al. [200], was chosen for folding assays in the presence of soluble chaperones as it had been reported to be folding competent in urea concentrations as low as 1 M, in stark contrast with the high urea concentrations required for efficient folding of HT PagP [200,211].
For example, anandamide at a low concentration makes the blastocyst competent for implantation, while at a higher concentration it attenuates this response [16].
Nonjaundiced controls were assumed to represent either heterozygous or wild-type Wistar rats that both are UGT1A1 competent and possess unconjugated bilirubin concentrations approximating 1 μM.
A study of 218 subfertile men found that their inhibin B concentration accurately (95%) differentiated between competent and impaired spermatogenesis, while the FSH concentration was less accurate (80%) [ 5].
These findings are in agreement with our other experimental findings [16], as well as our mathematical modeling predicting that high concentrations of free (monomeric) signaling-competent Gαo-GDP are produced upon activation of Go-coupled GPCRs [33].
Donor DNA was added at various concentrations to 500-µl aliquots of a competent cell suspension.
Higher concentration and lower sampling rate make the LCT more competent to resolve non-stationary signals.
LPS concentrations of 1 ng/mL are sufficient to stimulate immune-competent cells.
The recombinant plasmid was propagated in Escherichia coli DH5α competent cells and purified with the Plasmid DNA kit (BioKit, Taiwan); the concentration of the purified DNA was determined in a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific Inc., USA).
Furthermore, extrapineal melatonin produced by local immune-competent cells acts in a paracrine manner as anti-inflammatory mediator in higher concentrations (mM range) [32 34].
