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The DNA plasmid, pUC19, was transformed into DH5α competent cells.
Plasmid DNA (pNeoβGal) was transformed into DH5α cells (competent cells).
15 μl of the elution was transformed to 100 μl of E. coli competent cells.
The obtained plasmids were used to transform chemically competent cells of Escherichia coli M15[pREP4] strain.
Frozen competent cells were prepared and transformed according to the established protocol44.
The transformation efficiency of competent cells can decrease during storage because of their low viability.
Competent cells must be stored on ice the whole time until heat shock.
Add 10ul ligation product into 50ul TOP10 competent cells, following standard transformation protocol.
In addition to these services, MacroLab can provide reagents such as competent cells.
The linearized recombinant plasmid pPICZα-vMIP-II-IgG3-TfN was transformed into X33 competent cells.
C41 strand competent cells Escherichia coli were used for protein expression as they are specialized in expressing toxic proteins (Novagen).
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