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The ligation was carried out at 22°C for 30 min and the product was transformed into E. coli DH5 α competent cells as per the TSS protocol [ 21] and plated onto LB agar containing ampicillin (100 μg/mL).
10 ng of a pcDNA3.1-CD11b pcDNA3.1-CD11bechnologies, Paisley, UK) was mutated to contain one of four ITGAM vectorts by using the PCR-based QuikChange II XLifetechnologiesMutagenesis kit (Agilent Technologies, StockPaisleyK), followed by transformation of XL-10 Gold UKtra-competent cells, as per the manufacturer's instructions.
Frances Starr is competent as Mrs. Leeds.
Vera Gordon is competent as Benny's mother.
He seemed at least as competent as, say, a Red Foo.
No, they're not as competent as bees.
He also needs to make his second-term cabinet as diverse and competent as possible.
Our federal government is only as effective and competent as its governors.
I began musing about my capacity to live successfully with someone as competent as Louise.
"Susan Rice is as competent as anybody you will find.
You will become more and more competent as you practice.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com