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The zero minute control however provides a reference for the amount of DNA and the competency of the cells.
In many cases in yeast, small colonies reflect impaired respiratory function, so we examined the respiratory competency of the cells under starvation conditions using 2,3,5-triphenyltetrazolium chloride (TTC), which stains respiratory competent colonies red [14].
The transposition efficiency was calculated as the number of colonies resistant to kanamycin divided by the competency of the cells.
Competent cells were transfected with 10 μL of DNA and plated out on LB agar with 50 μg/mL kanamycin, and in dilutions on LB agar with 100 μg/mL carbenicillin to establish the competency of the cells.
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These authors suggested an interesting model in which Cdk activity is preserved at a low level during the DNA damage response, and such residual Cdk activity is required for maintaining the competency of the cell for checkpoint recovery.
As this can be accompanied by either apoptosis or autophagy – depending on the cell line – the secondary death mechanism must depend on the genetic competency of the cell line, and therefore the induction of either apoptosis or autophagy is not the determinant of sensitivity.
Mitochondrial DNA depletion causes an overall reduction in respiratory competency of the affected cell or tissue (Barthelemy et al., 2001; Sarzi et al., 2007).
Further insights about the immuno-competency of these cells in combating any infection or insult will help to identify a new function of these cells in non-professional antigen presentation in the injured CNS.
NYVAC was derived from Copenhagen strain by the precise deletion of 18 open reading frames encoding functions involved in the pathogenicity of the virus as well as in host-range regulatory functions governing the replication competency of the virus on cells derived from certain species, including human and mouse [17].
To better understand the kinetics of this observation, we monitored the respiratory competency of atg1Δ cells by TTC staining.
Yet, constant leaking of T cells and widespread DNA damage in surviving T cells must have consequences for the composition of the T-cell repertoire and the functional competency of individual T cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com