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This result is then also consistent with a role for IPC NB specification in closing the competence period in the pPIm.
After the expansion to the 8-cell stage, the cells entered a cell cycle arrest and formed a neurogenic placode; the pPIm then initiated a lengthy proneural competence period as the various NB identities were produced.
Although the IPC NB was ultimately specified, perhaps owing to a drop in transgene activity, this result suggests that the competence period for the IPC can be extended by at least 2 hours if Notch activity is maintained at a high level in the placode.
Thus, although the competence period to form an IPC NB ended near the time that the IPC NB normally first appears, Notch activity and neurogenesis in the pPIm placode persisted for at least 3 hours after this point, through the end of stage 14 (de Velasco et al., 2007).
A 3-hour upshift after 680 minutes of development produced no supernumerary IPC NBs; hence, the end of the competence period for pPIm cells to take the IPC NB fate occurs at 500 minutes of development, corresponding to the end of stage 12, when the IPC NB first expresses Dac.
Similar(55)
The maximum time larvae can survive in the water column (and still be competent to settle and metamorphose) affects the capacity for long-distance dispersal, and broadcast spawners have a wide range of maximum competence periods.
The Labour Party lost its reputation for economic competence in the period from 2008 to 2010".
Providers who do not conscientiously pursue a programme to maintain state-of-the-art practice could lose half of their competence in that period of time.
From a performance and clinical perspective, carbohydrate energy substrate plays a crucial role in exercise performance [43, 52], fatigue attenuation [12], and immune competence [53] during periods of exertional stress.
This is, in itself, practically unheard of and to have such a sustained period of competence seems as far off now as Wembley glory or the possibility of a good sit-com.
This contributed to their increasing confidence and competence in this early period of career transition.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com