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The colour compensations were performed according to the LightCycler 2.0 Software 4.1 manual and used in all further experiments.
The compensations were performed by using p.adjust stats) function of the R. The threshold used was 0.01 in a two sided manner.
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Two crosstalk compensations are performed by optimizing the PGCs to broaden the spectrum under bar-state and optimizing the two active MZIs to broaden the spectrum under cross-state.
Instrument settings and fluorescence compensation were performed on each testing day using unstained and single-stained samples.
Normalisation and compensation were performed on unstained controls.
Data analysis and software compensation were performed using WinList (Verity Software, Topsham, ME).
Finally, bidirectional motion compensation is performed again.
Then, motion compensation is performed between the side camera frames.
Similarly, color compensation is performed for color calibration in [11].
Note that no CFO compensation is performed in this paper.
The temperature compensation is performed by an on-chip DSP block.
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