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If necessary charge compensation was done using a Flood Gun (e- energy ∼6 eV/0.05 mA current).
The proper surface-charge compensation was done by fitting C-C, C-H component of C 1 s peak to reference binding energy 284.5 eV.
For data collected using the FACScan, compensation was done manually using single-stained cells.
Unspecific background of individual channels was determined with isotype controls and color compensation was done on single color-stained samples.
For data collected using the LSR II and FACS Canto, compensation was done with FACS Diva software using single-stained compensation beads.
Compensation was done using single-stained cells.
Similar(52)
Doppler shift compensation is done at each receiver separately.
The compensation is done by delaying the transmission of the synchronization messages.
This compensation is done for the frequency bins around the notch frequency.
Then, motion compensation is done by changing the reference block (see Figure 5).
Yet the signal is taken as a reference signal in the second stage where the phase compensation is done.
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