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One means of distinguishing is to test whether the polymer dissolves in a compatible solvent or merely swells without dissolving.
ESI-MS/MS analysis of the assays requires that the samples be dissolved in a compatible solvent free of nonvolatile salts and detergents.
The organic extracts of PM0.5 will be transferred to a compatible solvent (DMSO) and tested (24 h at 37°C with 5% CO2) in duplicate at increasing doses using A549 cells.
52 53 The PM0.5 organic extracts, dissolved in a compatible solvent (dimethylsulphoxide; DMSO), will be tested in duplicate at increasing doses with S. typhimurium TA100 and TA98 strains, which are generally utilised for environmental studies, 18 and NR98 and YG1024 strains, which are particularly able to detect nitrocompound.
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It is intriguing to consider whether these compatible solvents could be harnessed to sequester water from P. acnes.
The third protocol was adapted from a previously published protocol [ 42] to use LC-MS compatible solvents.
It was observed that with MS compatible solvents, silydianin has some peak fronting, which was improved using the shell core XB-C18 column compared to other columns [ 30].
Although there have been no reports of bacterial inhibition using compatible solvents, their use at high concentrations under conditions of low water activity has not been tested.
The dried sample extracts for the LC positive and LC negative mode were reconstituted in acidic and basic LC- compatible solvents.
It may well be that under these conditions even compatible solvents can be employed to reduce the water activity below a level permissible for growth.
All the process has been carried out by using commercial materials and industry-compatible solvents.
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