The molecular weight of Ct-0-GFP (37 kDa) was compatible with a fusion of GFP (26.9 kDa) and a 10-kDa Ct-0 fragment.
In support of this conclusion, mapping of ancestral centromeres by Gordon et al. [ 37] showed that C. glabrata CEN3 is an ancestral centromere and synteny is conserved across it, a result hardly compatible with chromosomal fusion of two different chromosomes.
Five main mechanisms for homothallism have been characterized in fungi: a) same-sex mating (monokaryotic fruiting), b) fusion of compatible mating type genes into one locus, c) unlinked compatible mating types genes present in one genome, d) mating-type switching, or e) packaging of two compatible nuclei into one spore (pseudohomothallism) [44].
A typical life cycle in the majority of basidiomycetes has a prolonged heterokaryotic (often dikaryotic) somatic stage, usually containing two genetically heterogeneous nuclei resulting from fusion of compatible homokaryons (Fig. 2a f), which is governed by a self-incompatible mating system (Anderson and Kohn 2007; Brown and Casselton 2001; Raper 1966; Raudaskoski and Kothe 2010).
Fusion of compatible haploid cells produces an extended and free-living dikaryotic hyphae with clamp connections.
However, haploid cells are not infective and only the dikaryotic hyphae formed by fusion of compatible sporidia can infect the host.
Following fusion of compatible partners, progression through the sexual cycle depends on a second compatibility checkpoint that relies on the formation of a heterodimeric homeodomain transcription factor (HD1/HD2) encoded also at the MAT locus [ 16, 17].
Several species in this genus have been well studied for the evolution of reproductive interactions (see, for example, Day, 1979; Le Gac et al., 2007a; Giraud et al., 2008), and cells are known to respond to the presence of the compatible mating type by halting the cell cycle and induction of conjugation tubes, resulting in fusion of the compatible cells.
His lumbar spine and sacroiliac joint films revealed fusion of the spine, known as 'bamboo spine' compatible with the diagnosis of ankylosing spondyloarthropathy.
This could either be ATP-dependent translocation of GBP across the PVM, or it could be ATP-induced fusion of GBP-containing vesicles with the PVM; the data is compatible with either model.
