Lychnis flos-cuculi is self-compatible, but its flowers are considered to be predominantly outcrossed [27], [28], [50].
This second bout of ethylene production is thus a good candidate for induction of senescence, and could possibly explain the morphology of flowers following compatible crosses two days post-pollination (Figure S1).
If compatible pollen entered the flower, the reasoning went, these enzymes, called S-RNases, were somehow cleared away before they could do any damage.
As N. attenuata is a self-compatible plant, anthers of flowers were removed in the morning between 5 and 7 am from experimental flowers, just before anthesis (Kessler et al., 2008) and 12 hr before flowers open and are accessible for pollinators.
This particular species of Platanthera is diploid and self-compatible with a flower morphology typical of plants adapted to hawk moth pollination (Sheviak and Bowles, 1986).
Since apples are self-incompatible, anthers from the compatible 'Royal Gala' were collected from flowers at an advanced balloon stage and left to dry on a paper at room temperature around 20 °C for 24 48 h until dehiscence.
The hermaphroditic, hummingbird-pollinated flowers are self-compatible (Ramírez and Ornelas, 2010), and ripe, lipid-rich, purplish-black fleshy fruits are dispersed by a variety of resident and migratory bird species (López de Buen and Ornelas, 1999, 2001; Ramírez and Ornelas, 2009).
Since apples are self-incompatible, anthers from the compatible cv Royal Gala were collected from flowers at an advanced balloon stage and left to dry on paper at room temperature around 20ºC for 24 48 h until dehiscence.
At 24 hours after compatible self-pollination (HACP), the P. inflata flowers appear quite normal but are condemned; at 36 hours the flowers are quite wilted [ 6].
Indeed, Rhizophora species have been shown to be self-compatible [ 27, 55, 56], possessing flowers that are mainly wind-pollinated but with facultative pollination by small insects [ 27, 57].
