Sentence examples for compatible entry from inspiring English sources

The genome editing tool suites contain two additional compatible entry vectors, pEntry A and pEntry B, for packing the sgRNA cassette (Fig. 1j).

All promoters were cloned by PCR into Gateway compatible Entry clones and finally inserted into the destination lentiviral vector by LR Clonase II recombination according to manufacturer's recommendations (Invitrogen).

We added the Gateway® recombination elements attB2 (forward primers) and attB3 (reverse primers) to the 5′ends of the genomic primers, to facilitate the cloning into Gateway® compatible Entry vectors.

This collection is contained in modular Gateway® compatible Entry vectors, is amenable for large screens and is publically available to the community through the at the DNASU plasmid repository (https://dnasu.org/DNASU/Home.do).do

A Gateway compatible entry clone for DreERT2 was constructed by PCR stitching of Dre (minus the nls) from plasmid DNA containing a codon-improved version of Dre (a generous gift of Francis Stewart, Biotechnology Center TU, Dresden, Germany) and ERT2 (Feil et al., 1997) separated by a short linker se quence (Hunter et al., 2005) to generate DreERT2.

The FAMA promoter (2.5 kb, [ Ohashi-Ito and Bergmann, 2006]) and full-length cDNAs of FAMA and RBR were cloned into Gateway compatible entry vectors, typically pENTR/D-TOPO (Life Technologies, Carlsbad, CA), to facilitate subsequent cloning into plant binary vectors.

Human POT1 coding sequence was obtained from Invitrogen's Ultimate ORF Clones collection in Gateway-compatible entry vector pENTR221-hPOT1.

These forward and reverse primers contain Gateway-compatible sequences to allow the cloning of the resulting promoter regions in Gateway-compatible entry vectors (Table S8 in Additional file 6).

Human cDNAs in Gateway-compatible entry clones were obtained from hORFeome V8.1 (Yang et al. 2011) and shuttled into yeast destination vectors (Alberti et al. 2007) using LR clonase II (Invitrogen) to generate expression clones.

Fully assembled TAL effector arrays and surrounding N-terminal and C-terminal regions were cloned into the gateway-compatible entry clone, pZHY013, using XbaI and BamHI (generating pMC89 95, pZHY013-TALN1 4). pZHY013-TALN1 4

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