Sentence examples for compatible ends from inspiring English sources

A protocol has also been reported for the cloning of four fragments using a restriction-ligation using type II enzymes that produce compatible ends, such as EcoRI and MfeI [27].

A 360 bp PCR product containing XbaI/MfeI compatible ends and a unique HpaI restriction site was ligated into XbaI/MfeI-digested pZErO-2E3-2.7 pZErO-2E3-2.7 pZErO-2E3-2.72E3Δ19k-HpaI.

These sequences were subcloned into a pSLfa plasmid [21], previously modified by digestion with BamHI and BglII, followed by ligation of the compatible ends to remove both restriction sites.

Both enzymes produce DNA fragments with compatible ends.

pUASTattB was linearised using BglII (Roche) to provide BamHI compatible ends.

The fragments were subjected to EcoRI digestion and circularized by ligation of the compatible ends, and subsequently randomly sheared.

To generate compatible end for the 35S insert vector SUB 3×myc pCAMBIA2300 was digested first with BamHI, made blunt with T4 DNA polymerase, and subsequently gel purified and digested with SpeI generating 35S::SUB:35Syc pCAMBIA235S.

Small DNA molecules (15 20 bp) containing one compatible end were ligated to the appropriate 'sticky end' of the restriction fragments.

In addition, the SIMPLE method can be adapted to insert any sized DNA fragment into a vector using a two-step PCR approach, and can be used to ligate any number of DNA fragments with non-compatible ends in the specific order desired.

The oligonucleotide linker primers oBB1443 and oBB1444 (HindIII-NheI-XbaI) designed to have BglII-compatible 5' ends at each end were annealed as described previously and ligated to BglII-digested, CIP-treated pT3TS to make pT3TS-linker (pBB231).

The oligonucleotides were designed to constitute a double-stranded DNA fragment containing the P AOX 1 promoter core region with EcoRI- and NheI-compatible ends when annealed for subsequent easy cloning into the pPPE20 vector.