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This allowed automatic identification of cytonuclear compartments, within which protein expression density was then calculated.
To identify the intracellular compartments within which VTI13 functions, we characterized GFP VTI13 subcellular localization in root epidermal cells and root hair cells using confocal scanning laser microscopy (CSLM).
It is especially important for drug discovery strategies to consider the different biological compartments within which each of these parasites resides in the mammalian host and the damaging effects that they exert.
In each cell 50 regions of interest (ROI = 131,065.74 nm) were then randomly placed in each of the selected cell compartments, within which the amount of gold particles was counted.
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The function of proteins is, in part, defined by the cellular compartment within which they reside.
Thus, tumour interstitial fluid (TIF) is an appropriate compartment within which to measure pathophysiologically relevant sHA concentrations.
The AQUA score of the target protein in each subcellular compartment was calculated by dividing the target protein compartment pixel intensities by the area of the compartment within which they were measured.
This requires hijacking of molecular complexes involved in membrane traffic; the pathogen can then invade a host compartment within which it can hide from degradation pathways and replicate in safety.
Although it is unclear whether any of these cells were viable, it appears that they were taken up into a nondegradative compartment within which they persisted for extended periods.
Thus identifying the compartment(s) within which each protein is located, i.e. its SCL, is an important step in understanding its specific role in cellular physiology, and indeed in modelling this physiology more generally.
Eukaryotic cells contain a number of physical compartments, across and within which their PPI networks are organised and structured.
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