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In this research, fibers possessing multiple compartments were prepared using the single-step electrospinning method and a modified non-concentric multi-needle.
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Prior to analysis samples from the SBS compartment were prepared by pipetting 2.0 mL of sample into a 50 mL volumetric flask using a "to contain" pipette.
Analysis of samples from the dialysate compartment was prepared by pipetting 3.0 mL of sample into a 10 mL volumetric flask using a "to deliver" pipette and adding 0.5 mL of HCl to the volumetric flask.
In the solution electrospinning compartment, the PLA and SF nanofibers were prepared by electrospinning a PLA solution (14 wt%) in chloroform/DMF (5/1, v/v) and an SF solution (7 wt%) in HFIP, respectively.
To quantitate target protein expressions in each cellular compartment, whole cell lysate, membrane, cytosolic, and nuclei fractions were prepared using differential centrifugation as we previously described [ 24].
Control plates were prepared in the same conditions, except the second compartment was filled with sugarless M medium complemented with an equal volume of DMSO only.
Dual drug-encapsulated nanoliposomes were prepared encapsulating ERL into lipid bilayer membrane and DOX into inner compartment of the nanoliposomes.
Free-standing membranes were prepared by electropolymerization on a gold sputtered microporous polycarbonate foil which was mounted in a two-compartment measurement cell.
The samples were prepared by incubating 2 µl mixtures on freshly cleaved mica for 3 min in a sealed compartment at room temperature.
Nuclear and cytoplasmic extracts were prepared from cells treated up to 12 h with rNef, and eEF1A, Exp-t and rNef were assessed in both cellular compartments.
were prepared.
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