To determine whether Bud1 is involved in the formation of the ER diffusion barrier, we assayed Sec61-GFP compartmentalization using FLIP in bud1Δ, bud2Δ or bud5Δ mutant cells.
The success of the enrichment experiment indicates that [FeFe] hydrogenase mutants can be screened in an extremely high-throughput fashion by in vitro compartmentalization using C12-resazurin to link enzyme activity to the development of a florescent signal.
Hower and Collins (1989) presented the first building block for an analytical model that detects reservoir compartmentalization using production performance.
Maximum likelihood phylogenies were evaluated for evidence of compartmentalization using four statistical tests.
Finally, given HIV's extraordinary evolutionary rates and the inherent difficulties in obtaining samples at the time of transmission, we investigated the relationship between sampling time and virologic compartmentalization using five distinct algorithms.
This estimation can also be used in confirming reservoir compartmentalization.
For the case of plant cell walls, for example, in theory NSOM could be used in a similar way to characterize the compartmentalization and distribution of cell wall matrix polymers, as well as chemically distinct microfribrils arranged in layers.
The sampling and quenching routine used in this work did not allow discriminating between compartmental concentration differences and, thus, compartmentalization was not accounted for in the dynamic network model.
The results obtained with the H. neapolitanus RubisCO replacement mutants used in our study strongly imply that some structural feature unique to carboxysomal RubisCO protein and absent from the noncarboxysomal species is required for compartmentalization into carboxysomes.
For non-clinical research purposes the size of the region used in our experiments is sufficient (more than 2 Mb) to extract safe conclusions about the local chromatin interactome and the compartmentalization of the genome.
