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To further highlight the importance of contemporaneous sampling in compartmentalization testing, we found a correlation between the number of days between PL and BM sampling and several qualitative measures of compartmentalization (Figure 4).
The 50.4 kDa monomer of Pab87 consists of two structural domains, an N-terminal PRP domain associated to a C-terminal lipocalin domain that plays a crucial role in the octamerization and in the active site compartmentalization (Figure 2A).
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Based on these identified Cd-responsive genes, we propose an integrated schematic diagram of the mechanisms involved in Cd tolerance and adaptation and a specific model for compartmentalization (Additional file 13: Figure S3), which may provide novel clues towards the characterisation of molecular mechanisms underlying Cd tolerance in barley.
10.7554/eLife.01883.006 Figure 4. Compartmentalization of Nup49-GFP and Nsg1-GFP depends on the sphingolipids, but not on BUD1.
10.7554/eLife.01883.004 Figure 2. Sec61-GFP compartmentalization depends on sphingolipids and Bud1 module.
"The compartmentalization: one large image living on the picture plane, with lots of other images scattered around," he said.
However, Figure 5B clearly shows compartmentalization of SGS, and closer examination reveals a network of lines (red arrows) throughout this structure, which look exactly like the folded graphene sheets previously reported by A. K. Geim et al.[25].
In contrast to the results with young animals, we observed that one year old, "tumor free" animals displayed a high frequency of hyperplasic thymi with disrupted compartmentalization in cortical and medullary regions (Figure 4A; data not shown).
This change was so profound relative to any other treatment that we were unable to discern the subcellular compartmentalization of lysosomes (Additional file 3: Figure S3).
Therefore, the compartmentalization of the GFP-tagged CbfA-CTD (Figure 7A) in D. discoideum cells was analyzed by fluorescence microscopy.
DOI: http://dx.doi.org/10.7554/eLife.06088.003 10.7554/eLiFigure88.004 Figure 1 figure supplement 1. Compartmentalization of the MT cytoskeleton is robust to changes in oocyte geometry.
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