Sentence examples for compartment were washed from inspiring English sources
For immunostaining, free-floating sections of the one compartment were washed in phosphate buffer and incubated with the primary antibodies to NeuN (mouse anti-NeuN, Chemicon Internat.
At the appropriate time after infection of the somal compartment, the somal and axonal compartments were washed once with phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde for 10 min. After removal of the fixative, the cells were washed two more times with PBS.
Following coincubation, epithelial and basolateral compartments were washed twice, followed by addition of 400 μL of warm HBSS at the basolateral compartments and 200 μL of 100 μg/mL FITC-conjugated Dextran 4000 solution in HBSS to apical compartments.
The compartments (somatodendritic and axonal) were washed once with phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA) for 20 min.
This latter phenomenon may explain the fact that Cu/NOTA-ML uptake, in opposition to retained signal after 2 h), did not correlate with PACE4 expression levels as the presence of high peptide concentration kept intracellular and extracellular compartment into equilibrium until cells were washed but predominantly allowed for efflux of unbound peptide.
Conditioned media from the apical and basolateral compartments were collected, cell monolayers were washed 2 times with Dulbecco's PBS without Ca2+ and Mg2+, and radioactive counts were then determined in media from the apical and basolateral compartments and the cell fraction.
It is very likely that mobile endogenous calcium buffers were washed out during whole cell recording, even from small compartments such as spines [28].
Eighteen hours after either application the explants were washed and fresh medium (without drug) was added to the basolateral compartment.
Therefore, it is very likely that mobile endogenous calcium buffers were washed out at the start of imaging, even from small compartments such as spines and presynaptic terminals [28].
