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In particular, the evidence for medial compartment surface area and volume increasing with increasing bodyweight was weak.
The lighter shaded region shows the 95% prediction interval for this model, illustrating the range of plausible future values under this model; the darker shaded region shows the 95% confidence interval for the regression line Fig. 4 Animal bodyweights regressed against knee compartment surface areas.
After 24 h (for migration) and 48 h (for invasion) incubation at 37°C in 5% CO2 humidified air, the cells were incubated in Calcein-AM (molecular Probes) for another 1 h at 37°C before removing the remaining cells in the upper compartment surface of the membrane with a cotton swab.
In combination with pre-embedding immunogold labelling and section penetration, the techniques could be used to determine absolute numbers of particles as well as LD values expressed as number per μm of compartment volume or per μm of compartment surface.
Our retrospective data suggest that quantitative CT peritoneography provides parameters associated with overall survival (compartment surface area and volume) and posttreatment GFR (compartment volume) in MPM patients undergoing IP chemotherapy.
The compartment surface area was defined as the sum of the interface areas of all compartment voxel sides facing noncompartment voxels, where the area of a voxel side is calculated by multiplying the image resolutions along the two directions spanning the plane at which the voxel side resides.
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Interestingly, however, unlike the wild-type protein, its localization to compartment surfaces depended on γ-tubulin.
Surprisingly, NOCA-1LICR+NHD required γ-tubulin to localize to compartment surfaces whereas full-length NOCA-1h did not.
Although compartment structure is disrupted in noca-1∆ worms, γ-tubulin::mCherry was still clearly observed on the compartment surfaces indicating that NOCA-1 is not required to recruit γ-tubulin to that location.
Consistent with this idea, a GFP fusion with the h isoform specific region localized to compartment surfaces, and this localization was dependent on a predicted palmitoylation site (cysteine 10; Figure 5 figure supplement 1).
This result explains why NOCA-1h localization at compartment surfaces was not eliminated by γ-tubulin depletion and implicates a potential lipid modification in providing a redundant means for NOCA-1 targeting to the membrane.
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