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The cell compartment filter selects minimotifs where both the ligand and its target are in the same cellular compartment.
Confluent monolayers were infected (from 0 4 h) with 1×10 E coli (a multiplicity of infection (MOI) 10 1) applied to the apical Transwell compartment (filter area 0.6 cm).
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For the first time, a one-compartment filter-press reactor with a boron-doped diamond (BDD) anode is used in the electrochemical degradation of E1.
The lower compartment of filter contains 500 μL complete EC medium with 20 ng/mL VEGF (R&D) as stimulator.
Approximately 5 × 10 human umbilical vein endothelial cells (HUVECs) (obtained from BCRC, number: H-UV001) were suspended in 200 μL complete EC medium (Gibco) containing 0, 5, or 12.5 μM CPP ecp and then seeded on the upper compartment of filter.
As EGFR is concentrated into intralumenal vesicles, membrane cargoes destined for a range of other compartments are filtered away into tubular elements (reviewed in [ 27]).
After 12 h incubation, the cells from the upper compartment of the filter were removed with a cotton swab, and then the cells on the lower surface of the filter were fixed in ethanol and stained with hematoxylin-eosin.
The filter compartment can be removed when the tea is to your taste.
The container, which is clear plastic and holds 50 ounces, has a perforated filter compartment for the tea, herbs or berries.
The filter compartment was filled with 200 g of lava grain inoculated with laboratory strains of ammonia-oxidizing bacteria.
The general PAMPA plate system consists of an acceptor compartment (96-well filter plate) and a donor compartment (96-well receiver plate) [37].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com