After a few hours, analysis using fluorescent dyes showed high rates of cell death in the lung compartment: The chip had metabolized the naphthalene, which then poisoned the lung cells.
(A ) Scanning electron micrographs of a multi-compartment PDMS chip.
Our results certainly implicate NFκB in mediating at least part of the phenotype in NDRG1 overexpressing cells, given our compartment fractionation and ChIP data.
Hybridization followed by mixing 30μl of the PapilloCheck® Hybridization buffer in a fresh reaction tube with 5 μl of the PCR-product at room temperature and transferring 25 μl of the hybridization mix into each compartment of the chip.
Imaging multiple compartments on the same chip allowed us to analyse Min oscillations in about 200 compartments under the same experimental conditions at the same time.
Available in black/red or brown, the suede playing field is accented with stitching; a zippered compartment holds die and resin chips.
Chips were provided with an RNA isolation compartment fitted with Rheonix proprietary silica membrane.
In the final version of the CARD, dry target-specific amplification reagents will be provided to the amplification compartment, a replaceable 0.2 mL microtube affixed to the bottom of the microfluidic chip.
They took naphthalene, the smelly ingredient in mothballs, and ran it through a three-chambered chip containing a rat's lung and liver cells and an empty compartment representing the rest of the body.
The acquisition setting was selected such that the dynamic range of the CCD chip was not exceeded, and an optimal contrast of the endolysosomal compartments with respect to the background was achieved.
Chip designs can be classified according to the method of creating isolated compartments, with either solid borders materialized by microchambers, microwells, microvalves, etc., or fluid borders, in particular, microemulsions 13.
