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The estimated sequence accuracy of the total repeat region was 99.8% based on a comparison with the reference sequence.
This procedure has been dubbed "resequencing," since it re-reads multiple homologous sequences in comparison with the reference sequence.
Furthermore, because complementary RNA probes are designed based on reference genome sequences, we were limited to find variants in comparison with the reference sequence.
De novo assembly of individual genomes followed by comparison with the reference sequence was a key method used to discover such sequences.
This observation is supported by previous studies using oocytes that revealed significantly reduced atorvastatin uptake by OATP1B1*15 in comparison with the reference sequence [ 46].
In comparison with the reference sequence (DQ869678), the HvNAM1 in this study had five SNPs, located on bases 234, 544 and 1433 in cultivated barley, and on bases 544, 1190 and 1427 in Tibetan wild barley.
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The output files presented a base call for each nucleotide position and allowed a direct comparison with the reference sequences obtained by Sanger sequencing.
We tested this approach on the wheat chromosome 3B dataset but it did not detect any of the chimerical contigs identified by comparison with the reference sequences.
Reference genome sequences for several crop species are now available, which permits both rapid identification of candidate genes through bioinformatic analysis and SNP discovery through comparison of the reference sequence with ones of various cultivars (Edwards and Batley 2010; Kim et al. 2010).
The comparison of Asian and African human genomes with the reference sequence has revealed 5 Mb of novel sequence in each assembly containing population-specific coding regions [ 14].
However, no sequence variations were found in comparison to the reference sequences.
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CEO of Professional Science Editing for Scientists @ prosciediting.com