Sentence examples for comparison to normalization from inspiring English sources

Expression of COX-2 mRNA levels per mg tissue is most simple in comparison to normalization with respect to the housekeeping genes B2M or GAPDH.

In comparison to normalization through conversion of read numbers to tags per million (TPM), median-normalization accounts for extreme cases, where a few highly differentially expressed genes reduce the amount of sequencing reads for other transcripts exceptionally in one of the compared libraries.

Indeed, in comparison to other normalization methods we obtained the largest number of differentially regulated genes when the MAS5 normalization was used.

More precisely, this ratio (R) was calculated taking into account the real-time PCR efficiency of each gene (E) and the crossing point difference (ΔCP) of a test condition (IE, LE or L1), as compared to the reference condition (EE, IE or LE according to the comparison (EE-IE, IE-LE, LE-L1)), and expressed in comparison to the normalization gene (actin) using the following model [ 77].

Adding mappability as a normalization parameter results in a substantially reduced total number of bases called in comparison to GC content normalization - calling only 25% of the total base pairs outputted by GC normalization but going as low as 6-76-7% three genomes (CEU, YH1, and KOR).

The normalization of cDNA using DSN was very simple and highly efficient in comparison to other cDNA normalization methods [ 21].

The simulation study provides strong evidence that our weighted quantile approach yields up to a 10%% gain in power in comparison to the conventional normalization method.

The Bioconductor package edgeR [ 17, 137– 139] was used for single factor, pairwise comparisons to calculate normalization factors, estimate tagwise dispersion and determine differential expression (DE).

The up-regulation of NRG1 during dysplasia was much smaller, when GAPDH was used for normalization in comparison to the other reference genes.

For var transcriptional analyses, var gene normalization was done by comparison to adenylosuccinate lyase (PFB0295w).

For calculation of the χ values, original library sizes were adjusted with a median-based normalization factor for each comparison to account for potential outliers.