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b Comparison of radioactivity concentration in brain determined by resection just after SPECT (CBrain-sampling) and that by SPECT 15 57 min post-injection of 99mTc-HMPAO (CBrain-SPECT).
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Comparison of radioactivity- and fluorescence-based assays showed that the two are equivalent, indicating no interference by the fluorescent tag.
Some of the limitations of high-resolution autoradiography can be overcome by developing automated 3-D reconstruction procedures from large series of sections from the same brain [6], [7], [8], [9], [10], [11] and by then allowing comparisons of radioactivity distribution across regions between animals belonging to the control or experimental group.
b Comparison of the radioactivity concentrations in sampled whole blood (ex vivo) vs. the image-derived heart concentration from the PET scanner.
c Comparison of integrated radioactivity concentrations in arterial blood samples (AUCBlood-sampling) and that in the heart on planar images (AUCBlood-planar) until 19 s post-injection of 99mTc-HMPAO.
Furthermore, a comparison of the radioactivity-based and fluorescent-based assay revealed a very high correlation of 0.93 in control subjects and 0.91 in case patients.
Comparison of the spiked radioactivity with the radioactivity measured by LSC yielded recovery of the extraction steps and comparison with the radioactivity measured on the HPLC yields overall recovery (Table S1, Supporting Information).
Comparisons of organ radioactivity concentrations (%ID/g) and TORs in the biodistribution studies or %ID/cc in PET imaging studies between the two radiotracers were performed using separate unpaired, two-tailed Student's t tests, matched organs and time points.
A comparison of the PET radioactivity counts in the whole brain is shown in Fig. 3.
The SA of [11C]MMPIP was calculated by a comparison of the assayed radioactivity to the carrier mass which was measured from its UV peak at 254 nm.
The peptide yield for each unnatural amino acid was determined relative to the peptide yield for the corresponding natural amino acid, by comparison of the peptide radioactivity for the all-natural peptide with that containing the unnatural analog.
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