The first step would be a comparison of pooled samples representative of the disease to normal subjects or a comparator disease.
While several tools for analyzing Pool-Seq data of single populations are already available (Bansal, 2010; Kofler et al., 2011; Pandey et al., 2011), to our knowledge no standalone software tool is available for the comparison of Pool-Seq data for multiple populations.
The comparison of pooled data obtained with all treatments did not show significant differences between the groups of controls and hypersensitive subjects.
Comparison of pooled data for Kandy, Kurunegala and Ratnapura districts with data from Epidemiology unit also show similar patterns indicating that the study received representative samples.
Interestingly, comparison of pools and individual worms confirmed the diagnostic potential of G6PD to distinguish worms from KBp in Khon Kaen versus KLp and KBp versus the two isolates from Laos.
A significant positive correlation was observed for the entire cohort, but correlations were clearest upon comparison of pooled data from the bottom and top third of APOBEC3B expressing patients.
Although the analysis using the four fruit types is biased for WLT, the comparison of pools and Hz-Od experiments has identified a group of genes (k-means 11; Figure 6A) that only respond to cold in Hz fruit, and thus are good candidates to be related with Hz phenotype.
The implications of the present study in relation to the design of biomonitoring programmes are: (1) care should be taken to ensure an equal/constant sex composition when making interannual comparisons of pooled samples.
Comparisons of pools and individual worms showed that for the 10 enzymes examined the multiple bands detected in pools were not due to multiple loci.
Genome-wide scans with an Affymetrix high-density single-nucleotide polymorphism (SNP) microarray enabled replicate comparisons of pools of different molecular forms, in three separate populations.
Univariate and multivariate comparisons of pooled data were performed using a generalized linear model with a binomial distribution and logit link function.
