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Comparison of expressions within the same sample in TSP and k-TSP helps to eliminate the influence of sampling variability due to different subjects.
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Fig. 6 Comparison of expression levels of dhaD of the strains E. coli BL21 DE3 pLysS (DE.), E. coli BL21 DE3 pLysS/dhaD (DE3/dhand and K. pneumoniae SRP2 (SRP2).
In this paper we describe a novel two-step method for comparison of expression of alternatively spliced genes by quantitative PCR (QPCR) applying different primer pairs.
The RNA capture/QPCR method we described here can be used for quantifying the expression ratios of alternatively spliced mRNAs from a single gene or for direct comparison of expression of different genes.
Quantitative comparison of expression used an upper and lower limit factor of 0.5 (2-fold).
Instead the new probesets enable comparison of expression levels within and among the resulting transcripts.
In addition to that, the HapMap expression data allow us to perform a direct comparison of expression levels between individuals.
Additionally, a cross-species comparison of expression patterns revealed that Plasmodium-specific genes exhibit significant expression divergence.
Again, this combination improved the standard protocol for comparison of expression patterns of mRNA and proteins within the same sample.
To date, assessing cross-species conservation of gene expression using microarray data has been mainly based on comparison of expression patterns across corresponding tissues, or comparison of co-expression of a gene with a reference set of genes.
Under appropriate conditions, the relative change in OD is a reliable indicator of changes in mRNA expression allowing for comparison of expression of specific mRNAs under different conditions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com