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Additionally, compounds that exhibited MIC < 64 μg/mL are considered active and could be used for a direct comparison of activities between compound classes [ 9] although a more recent review by the same author suggests that only compounds displaying MIC values < 10 μg/mL might be considered of interest to the pharmaceutical industries [ 31].
It is the first expression system which enables direct comparison of activities between enzymes or variants from the supernatant.
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Finally, our results revealed that the HAC1 RNA cleavage activity of yIre1 is intrinsically more active than RIDD, and thus, comparisons of activities between different forms of IRE1 should be performed carefully.
Besides, for a fair comparison of activity between native enzyme and CLEAs, the enzymatic protein content of both of them should be the same.
The comparison of activity between guessing and passive observation is rendered more difficult by the fact that they were acquired in separate sessions, and results should be considered with care.
The lack of activity on DR5-mediated A2780 and Jurkat cells and the comparison of activity between BJAB and BJAB DR5-deficient cell line with rhTRAILWT clearly confirmed the DR4 selectivity of the variants 4C7 and 4C9.
The extent of neurogenesis was assayed in Stage 19 embryos by in situ hybridisation (ISH) to detect neural β-tubulin (Fig. 4A, B), allowing a semi-quantitative comparison of activity between proteins.
In this context, specific activities appear as the key for guaranteeing that comparisons of activity between free enzyme and CLEAs are done based on a similar amount of protein.
Secondly, a comparison of activity levels between GGG and non-GGG days was performed to evaluate the impact of GGG on girls' activity.
Finally, comparison of activity levels between GGG and non-GGG days was performed for each age group (i.e., 5-7 years, 8-10 11-13 years3 11-13 years
This method allows us to make legitimate comparison of activity rates between experimental groups in the DG, but, as pointed out by the reviewers, the comparison of the reactivation rates to the corresponding activation rates may be affected.
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