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Two solid-phase binding assays were designed and evaluated for their potential use in comparing the affinity of peptides to the Src SH2 domain.
Introduction of an additional CH3 moiety in 2-position led to a slight decrease of GluN2B affinity as can be seen by comparing the affinity data of cis-4a and 5.
Protein arrays are optimal candidates for comparing the affinity of an analyte for different ligands.
Enrichment of the target region was determined using a real-time PCR detection system (MyiQ, Bio-Rad Laboratories, Hercules, CA) by comparing the affinity purified sample (anti-GFP) with the negative control (rabbit serum).
Interestingly, a much larger change was observed when comparing the affinity of M2-1 for the negative F gene end (Kd ∼ 1 µM), which contains a single adenosine in position five, and the poly U, C, or G sequences (Kd >100 µM).
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To confirm that the 52+2 mutation does not affect hapten binding, surface plasmon resonance analyses were performed comparing the affinities of 52+2 Cys variants with their parent antibodies.
Expressing this in biological terms, this can be interpreted as comparing the affinities of 20 different tRNA synthetases for aminoacylating a given tRNA gene.
To this end, receptor specificity was analysed by comparing the affinities of LVT, dLVT and DOTA-dLVT in cells transfected with all of the members of the OT/AVP receptor family.
Biased signalling can be identified by comparing the affinities of ligands in β-arrestin recruitment assays with a G protein-mediated response such as vasoconstriction (MaG protein-mediated).
Commonly used florescent probes include: warfarin, phenylbutazone for site 1; ibuprofen, naproxen for site 2 and digitoxin for site 3. Miklós Poór et al. compared the affinity to HSA between flavonoids and warfarin [145].
We searched for signs of multiple interactions occurring simultaneously and compared the affinity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com