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Each sample protein concentration was determined by comparing its absorbance against a calibration curve, obtained from 0 to 8 μg samples from a 2 mg/mL BSA standard diluted in PBS.
The quantum yield of bimane was calculated by comparing its absorbance and fluorescence intensity at 360 nm to those of quinine sulfate, a common fluorescent standard that has a quantum yield of 0.54 in 1 N H2SO4, using the following equation.
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The reaction product was identified by comparing its retention time and its absorbance spectrum (200 400 nm, Fig. 5).
Each reaction product was identified by comparing its retention time and absorbance spectrum with authentic samples or isolated compounds previously characterized.
Furthermore, we have thoroughly validated our method and compared it to UV absorbance measurements.
Cell viability was determined by comparing the absorbance of samples to a standard curve [21].
The percentage inhibition of superoxide generation was evaluated by comparing the absorbance values of the control and experimental tubes.
The cell viability was obtained by comparing the absorbance of cells cultured on the nanofiber scaffolds to that of the control well containing DMSO.
The scavenging activity was determined by comparing the absorbance with that of the control containing equal volumes of DPPH solution and ethanol.
The cytotoxicity was measured by comparing the absorbance (595 nm) produced by the control cells and the absorbance produced by the cells exposed to different concentration of the lyophilized extracts (500, 250, 125, 62.5, 31.2, 15.6, and 7.81 ppm).
In experiments comparing the absorbance signal in undiluted serum samples from infected and non-infected patients, a signal ratio (absorbance in samples from infected subjects/absorbance in healthy volunteers) of 1.39 1.59 was observed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com