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We tested this hypothesis by comparing binding of sumoylated and free HDAC1-FLAG to GST-CoREST using GST pull-down assays (Figure 6A).
Tumor specificity of antibody cultures was evaluated by comparing binding of antibodies from these cultures against multiple melanoma cells (A-375, SK-MEL-2, WM-115) versus normal cells (Figure 5, A).
This is consistent with data comparing binding of multiple different SIMs to both SUMO1 and SUMO2, which suggests that negatively charged amino acids surrounding the hydrophobic core contribute to SUMO1 binding, but much less so to binding of SUMO2 [24].
(C ) Gel-shift assays comparing binding of P-labeled RRE-stem II or IIB40 to Rev. Free, F, monomer, M and dimer, D complexes are indicated.
When comparing binding of ETS and Runt family members to Kaiso sites in GM12878, we again found that members of both families co-localized with Kaiso, with RUNX3 and PU.1 having the highest enrichment.
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To distinguish between these models, we directly compared binding of MW1 and 3B5H10 to normal and expanded polyQ repeats within huntingtin exon 1 fusion proteins.
We recently compared binding of 111In-DOTA-JR11 111In-DOTA-JR11 111In-DOTA-JR11to ZR75–1 and 111In-DOTA-Tyr3-octreotate 111In-DOTA-Tyr3-octreotate 111In-DOTA-Tyr3-octreotate 111In-DOTA-Tyr3-octreotatenalizatoon assay to investigate differences in SSTR agonist and antagonist binding to cell lines with endogenous and exogenous SSTR expression.
Far-Western analysis was used to compare binding of WRN488 1432 and WRN239 499 to p300.
To address this question step by step we compared binding of LRRK2 mutants with ARHGEF7 in vitro.
In addition, we compared binding of peptide-free and peptide-loaded DR1 using an equilibrium sandwich ELISA assay (Fig. 4E).
To determine if both binding sites contributed equally, we performed a quantitative experiment to compare binding of the different forms of LITAF to Itch WW domains.
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