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All PCR products were sequenced and compared with the reference sequence.
If coverage was complete, the assembled sequence was compared with the reference sequence to determine if the clone were acceptable.
The obtained sequences were compared with the reference sequence of the National Center for Biotechnology Information (NCBI) Entrez Nucleotide database (gb/EU375803.1 Merkel cell polyomavirus isolate MCC350 and gb/EU375804.1 Merkel cell polyomavirus isolate MCC339), using the NCBI blast program.
and compared with the reference sequence for SALL2 (NM_005407.1).
This revealed a mutation (C493T, Thr160Ile) compared with the reference sequence.
Genome sequence data were compared with the reference sequence GRCh37/hg19.
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All the sequences that showed more than one difference from the mtDNA consensus sequence were compared with the reference sequences in GenBank using the National Centre for Biotechnology Information (NCBI) BLAST search.
Mutations or polymorphisms were compared with the reference sequences.
Sample sequences were compared with the reference sequences from GenBank to identify sequence variants.
The sequencing results were analyzed using Chromas 2.33 and compared with the reference sequences in the NCBI database.
Sequences were compared with the reference sequences (NM_000525.3 and NM_000352.3) using Mutation Surveyor v3.24 software (SoftGenetics, State College, Pennsylvania, USA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com