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The length of the tested contigs and corresponding pred-CA contigs were compared using custom Perl scripts.
SNVs from both platforms were combined into CG testvariant format and compared using custom perl/python scripts.
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Lists of genes were compared using a custom Java application written in-house.
Variants were annotated and sample calls were compared using our custom cloud based analysis platform seqplorer (http://www.seqplorer.org) (De Wilde et al., in preparation).
Homologous RAD clusters from the dominant and recessive lines were then compared using a custom k-mer matching algorithm permitting exact sequence matches (monomorphic loci), single mismatch (one SNP per read) and two nucleotide mismatches (two SNPs per read) per 28 bp sequence.
S. aureus gene expression changes between adherent bacteria and those arising 2 h and 6 h after cellular internalization were compared using a custom-designed and validated S. aureus oligoarray [ 53].
The miRNA expression in adenocarcinoma and squamous cell carcinoma cases was compared using a custom-made two-channel oligo-array of 713 human, mammalian, and viral mature antisense miRNAs including 2 internal controls with 7 serial dilutions starting at 5 μ and ending at a final dilution of 0.0016 μ (http://madb.nci.nih.gov/gal_fi les/CCDTM-miRNA700-V3px-A.gal les/CCDTM-miRNA700-V3px-A.gal les/CCDTM-miRNA700-V3px-A.gal les/CCDTM-miRNA700-V3px-A.gal
The mRNA expression profiles of the different treatments described above were compared using the Affymetrix GrapeGen custom GeneChip™ (Lijavetzky et al., In preparation).
Genotypes derived from exome data and orthogonal genotyping assays were compared using PLINK [ 24] and custom scripts.
Sequences were compared using BLASTX to the GenBank non-redundant database and to a custom database consisting of published chloroplast genomes.
Individual reports were parsed into spreadsheet format to compare using a custom Perl pipeline.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com