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To determine whether mutation can play a role in microcolony initiation, biofilm growth of P. aeruginosa PAO1 WT, ΔmutS, ΔmutL and complemented mutant strains were compared using confocal scanning laser microscopy (Figures 2 and 3).
One novelty of the present study is that by means of a parallel randomization, prospective and masked design, the two treatments were also compared using confocal microscopy.
To find out whether DSBs are formed in SCs, we compared using confocal microscopy the expression pattern of γH2AX, a DNA damage marker, in SCs challenged with AdcD1 and ionizing radiation (IR).
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The corresponding formed layers were compared using FESEM, confocal microscopy, TEM and TFXRD, pre- and post-SBF immersion.
Interestingly, in the presence of serum, we found an increase in the number of nuclear localized Bcl-3 in CYLD−/− cells compared to CYLD+/+ MEF cells using confocal microscopy (Figure 2F).
The HTS suitability of this assay was initially demonstrated by screening a marine natural product library, where the outcomes obtained using confocal imaging were compared with the alternative whole well fluorescent intensity readouts.
Patterning concentrations were determined using confocal microscopy by comparing the fluorescence intensity at a point 200 μm below gel surface against a standard curve relating fluorescence intensity to that of gels swollen in known concentrations of the patterning agent (see the Supporting Information, Figure S11).
In HeLa cells, similar cellular distributions of GFP galectin-1 fusion protein (GFP galectin-1d with those ofusionctin-1 (red) were observed using confocal microscoprotein
Storage Ca2+ levels were also analyzed in BA treated and non-treated cells by comparing Ca2+ storage emptying in response to 100 µM thapsigargin using confocal microscopy [39].
To rule out artificially high similarity scores, we compared the mitochondrial localization of wt-, G39- and G80-cofilin in COS cells using confocal laserscan microscopy (Supplementary Figure 2).
Consistent with the results obtained using confocal microscopy, the p32 siRNA-treated cells contained predominantly small spherical mitochondria, compared with the mainly normal elongated mitochondria in control cells.
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