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Parametric data were compared using an unpaired t test.

For continuous measurements, results were compared using an analysis of variance (ANOVA) and Newman Keuls post hoc test.

Results were compared using an unpaired, one-tailed t test.

Categorical data were compared using an unpaired Student's t test.

Nalbuphine vs placebo was compared using an unpaired t-test, as these two groups were independent.

Two path costs are compared using an if-statement, assumed to count one instruction.

The length variable (BCB) was compared using an analysis of variance (ANOVA).

Differences between groups were compared using an ANOVA, p values < 0.05 were considered statistically significant.

Variables were compared using an ANOVA, including genotype, treatment and time of study as factors.

P-TEFb-associated RNA levels were compared using an amplification efficiency value of 70%.

The slop of the curves was compared using an ANOVA test of regression.

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