However, compared to transformed tumor cells, CAFs are considerably more genetically homogeneous and thus represent an attractive target for cancer therapy [8].
Positive segregants from two T1 lines studied in detail showed reduced MSH7 expression when compared to transformed controls and null segregants.
In gene microarrays, 4 of 5 datasets showed stronger mean expression of NURR1 in normal breast as compared to transformed breast.
Higher levels of total soluble protein (approximately 1.4-fold) were secreted by the E. coli cells transformed with pET-CV2935 and cultivated in TB compared to transformed cells grown in LB medium and incubated under the same conditions.
This approach allows us to work in a carefully controlled experimental setup while still using primary tissues (mimicking in vivo conditions) as compared to transformed and immortalized cell culture lines where we could not be sure of the physiologic relevance.
Several transcripts encoding enzyme activities in both these routes (phosphoenolpyruvate carboxykinase, phosphoenolpyruvate/phosphate translocator, and malic enzyme, Fig. 5) were up-regulated (3 to 9-fold) in WRI1 expressing leaf tissue as compared to transformed control.
Differential gene expression is given as log2 of ratios of RPKM in leaves expressing the WRI1 homologs as compared to transformed control (log2 ratio of +3 means that a transcript is 2 = 8 times higher expressed).
The FA composition of TAG changed upon expression of WRI1 homologs with lower proportions of 18 0 and in general higher proportions of 18 1, as compared to transformed control (Fig. 3a).
Upon expression of WRI1 homologs in the N. benthamiana leaf tissue, notable differences in the number of differentially expressed genes as compared to transformed control were observed, but all five homologs induced a similar pattern in core metabolic pathways.
Transcripts showing a differential expression as compared to transformed control with a P-value close to zero (<10−307) were extracted to an individual table (Additional file 6 with log2 ratios in bold font) in which also differential expression values for all other leaf samples were included (even though having a P-value above this threshold, log2 ratios in italic font in Additional file 6).
