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Compared to their differentiated progeny, stem cells are often characterized by distinct metabolic landscapes that emphasize anaerobic glycolysis and a lower fraction of mitochondrial carbohydrate oxidation.
We showed that L1TD1 is highly enriched in undifferentiated hESC compared to their differentiated derivatives, indicating that it may be a useful marker to identify undifferentiated hESC.
In addition, undifferentiated mESCs appear to have a higher level of global histone acetylation with transcriptional hyperactivity as compared to their differentiated derivatives.
S-MAP identified 350 non-redundant up-regulated modules with reproducibly higher expression in stem cells compared to their matched differentiated cells across the compendium (<5% recurrence FDR).
Proliferative quiescence is characteristic property of some stem cells, which, as compared to their more differentiated progenitors, undergo infrequent divisions (Moore and Lyle, 2011).
We observed 2-fold more differentiated methylated cytosinces (mCs) in domesticated silkworms as compared to their wild counterparts, suggesting a trend of increasing DNA methylation during domestication.
Some data suggest that oncogenic mutations lead to loss of the ability for cells to maintain their differentiated state.
In vitro analysis showed that both SC and MSC adhered significantly better to PHB in the presence of fibrin and cells continued to maintain their differentiated state.
For rLSECs, immunostaining for the sinusoidal endothelial marker (SE-1) provided a convenient method to verify their differentiated state.
In their differentiated state, the NB cell lines and tumorspheres show lower PES1 expression.
Cells are maintained in their differentiated state by selectively activating some genes and silencing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com