Additionally, the silenced gene signature was more pronounced in ISS stage III MM compared to stage I and II.
Also, according to the study's results, LINE methylation levels were higher in stage II disease compared to stage I.
We found Chk1 to be highly expressed in stage I progenitors when compared to stage IV cells, and to steadily decrease as the cells differentiated (Figure 7A).
In the Chin gene profile, containing 118 frozen primary breast carcinomas, NTSR1 was found over expressed in stage IV carcinomas as compared to stage I with p = 0.003.
This data, although preliminary, indicates that stage I USC is genetically similar to stage I EAC-G3 compared to stage I EAC-G1.
Bmp2 and Bmp4 mRNAs were induced 1.7-fold and 3.0-fold, respectively, in stage 4 R explants exposed to Wnt3A (2x) compared to stage 4 R explants cultured alone (Fig. 6).
The H3K27me3 target genes were also overrepresented among genes underexpressed in MGUS patients (Figure 1B) and strongly associated with decreased expression in ISS stage III MM (Figure 1C), compared to stage I and II (Figure 1D).
Importantly, the suppression of the genes found in the profile was more pronounced in the advanced stages (ISS stage III compared to stage II and I) of MM progression.
Stage III and IV (1.70) had much shorter survival compared to stage I and II.
Stage III disease was, compared to stage IV disease, associated with longer progression-free survival.
In general, gains were more frequently detected in stage IV compared to stage I III tumours.
