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We have previously observed similar differences in Bacteroidetes content in male rat fecal samples subjected to 16S rRNA pyrosequencing compared to determinations based on qPCR or shot gun metagenomics [ 50, 64].
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Tregs also increased significantly in 7/8 recipients after the three post-transplant immunizations as compared to determination at 90 100 days post-transplant (P<0.05, paired t-test; Fig. 6B).
Compared to determination of allele frequencies in individual pools, it is more important to learn whether the allele frequencies among pools and sub-pools are significantly different from each other.
Similar results are obtained for other hydro-organic mixtures and compared to previous determinations using other methods.
Thus, the Delphi method may include and consider the process of reasoning compared to the determination of the solution using, for example, a decision method such as voting.
If such non-integrated viral DNA was abundant in cell cultures transduced with the d2 × 250 bp and the 1.2 kb.CMV vectors, titer determination using real-time PCR 3 days after transduction would overestimate the titers, compared to titer determination conducted 7 days post-transduction, where non-integrated viral DNA will be more diluted due to ongoing cell division.
Similarly, the RightSpotpH™ indicator reading was compared to the determination of aspirate pH by the external laboratory pH electrode.
Finally, MALDI-TOF MS more rapidly identified reduced susceptibility to caspofungin or triazoles in Candida and Aspergillus species, as compared to classical determination of the minimum inhibitory concentration [39, 73, 74].
In preliminary experiments, cell counting was compared to photometric determination with dyes WS1 and MTT.
Compared to experimental determination of Kp values, the use of in silico based algorithms such as tissue composition based models (e.g., Rodgers and Rowland) or correlation models (e.g., Yun and Edginton) have decreased the time and cost associated in the development of whole-body PBPK models.
I have a similar sense that at major research institutions with large IRB staffs of Certified IRB Professionals (CIPs) (http://www.primr.org/certification.aspx?id=206) and others, it is indeed often such a staffer, rather than a voting IRB member, who makes the exemption determination (as compared to expedited review determinations, which the regs required be made by one or more IRB members).
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CEO of Professional Science Editing for Scientists @ prosciediting.com