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The extended fasting effect was then defined as a contrast that compared time point 5 (36 hours fasting) with the average of the 4 baseline measurements (overnight fasting).
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Finally, a list of features significantly regulated at each time point was obtained using the same "limma" model and a set of contrasts, comparing time point samples versus controls.
To compare time point and time course-dependent phosphorylations between cell lines, the potency of target phosphorylation by X10 and IGF-I was derived as the fold increase in phosphorylation compared with that for insulin as referent.
To compare time points (T = 0 vs. T = 4) within the same subject, a paired t test with Bonferroni correction was used if data were normally distributed, or Wilcoxon signed rank test in case of non-normal distribution.
Post-hoc tests were also performed to compare time points.
An additional GEE post hoc analysis was made to compare time points.
Traditional analyses comparing time points two by two have serious limitations.
Eight models were significant at the 0.0025 level and underwent an additional GEE post hoc analysis to compare time points.
Comparing time points A to D an increase of IL-6, IL-8 was observed, comparing A to C: IL-6, IL-8 and CXCL1 increased.
However, in our case it is difficult to compare time-points from the different research platforms directly due to technical restrictions.
Scanning data from 10 runners were available for the analysis comparing time points t3-t1, t2-t1 and t3-t2.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com