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Four Lutjanidae snapper species with similar biology were compared from the same collection locations.
The design controls for unlinked segregating genetic variation because sibships are compared from the same introgressed lineage with and without the target region.
The disadvantage of mild trypsin treatment to isolate 'pure' ICM cells, is that TE and ICM cannot be compared from the same embryo and that the trypsin treatment by itself may cause a difference in gene expression.
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However, to ensure accurate comparisons, data were only compared from within the same laboratory (ie, Australia versus Australia and Qatar versus Qatar).
Second, I compared matrices from the same morph and different locations; and third, from different morphs.
The probe positions and the MR images compared are from the same children.
The control mice compared were from the same breeding but not always littermates.
Two trials were excluded from the pairwise analysis as they compared treatments from the same class (beta mimetics).
Three trials were excluded from the pairwise meta-analyses as they compared treatments from the same class.
We compared data from the same subjects in different conditions using one-factor ANOVA for group comparisons.
First we compared samples from the same community and found that the observed UniFrac false positive rate was consistently at or below the targeted 5% level (Table 3).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com