Additional studies comparing extracts from wild-grown fruit bodies with extracts from fruit bodies cultivated in vitro (under standard conditions) from the same strain will further elucidate the influence of strain characteristics on the metabolite profile and the respective bioactivities.
Matrix effect was evaluated by comparing extracts of spiked matrix with extracts of spiked blank matrix using water as blank matrix sample.
We found that all of these were suitable for comparing extracts from one individual, but varied too much to compare between individuals.
Using electrophoretic mobility shift assays comparing extracts from tumours and adjacent normal tissue, Wang et al (1999) demonstrated an activation of NF- κB in pancreatic cancers.
Recovery rates were determined by comparing extracted spiked plasma samples with non-extracted solutions of standards in methanol (IB 1.25, 5 and 12.5 μg/ml).
Furthermore, we tested for lateralization by comparing extracted signals from the regions of interest in the left and right TPJ (3-mm and 5-mm sphere around the peak voxel (−51 to 70 34) and (51 to 70 34), respectively).
a Person 1, b Person 2, c Person 3. To compare extracted candidates with a target, the target's model is used.
One-way analysis of variance (ANOVA) was used to compare extract's inhibitory diameters.
